Part:BBa_K597105
CCaS and CCaR Light Sensor System
CCaS and CCaR are integral proteins involved in the green light-induced gene expression signaling pathway as described in Tabor et al. CCaS is a surface protein which, in the presence of 532nm light, becomes photoactivated and begins autophosphorylation when in conjugation with a phytopsycobilin. The phosphate group is then transferred to CCaR, which becomes activated and can bind to Pcpcg2, a light-sensitive promoter, initiating downstream gene expression. CCaS and CCaR are thus a component of a light-induced gene expression system.
Cornell's intention with CCaS and CCaR is to ligate this composite with the Ho1, pcyA, and Pcpcg2 genes into a single vector that will function as a light-induced gene expression system. These genes, in addition to CCaS and CCaR, are required for light-sensitive gene expression, as they code for the biosynthesis of the phytopsycobilin.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 606
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2696
Illegal XhoI site found at 2252 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1270
- 1000COMPATIBLE WITH RFC[1000]
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